Mini grafts: alternative to obtain complete plants of Pinus patula propagated in vitro

Micro-grafting and mini-grafting trials were carried out in order to obtain complete Pinus patula plants generated vitro, derived from the fact that formation of roots micro-propagated shoots is very low or null. On other hand, there possibility generating clones with outstanding genetic characteristics. P. seeds established aseptic conditions geminated vitro DCR medium; aerial part was dissected base kept medium added 0.5 mg l-1 NAA maintain them served as mini rootstock, transferred 2.0 BA form seedlings an average 3.9 per explant at 30 days. 2-month-old nursery used rootstock for mini-grafts. For micro-graft tests, obtained taken apical meristems approximately 5 mm isolated conduct micro-grafting roots, case mini-grafts allowed grow a length 3 cm on grafted onto seedlings. In both cases, achievement materials evaluated measure growth had positive success. The response micro-grafts low, 10% success, addition manipulation being complex, which oxidation tissues. contrary, showed 93.3 grafting success 26.05 cm, two months after process